Production of Recombinant Hinnavin Ⅱ/MSH Hybrid in Insect Cells
2005
Bang, I.S. (Hoseo University, Asan, Republic of Korea) | Park, S.H. (Hoseo University, Asan, Republic of Korea) | Kang, C.S. (Hoseo University, Asan, Republic of Korea) | Bang, S.K. (Hoseo University, Asan, Republic of Korea), E-mail: skbang@office.hoseo.ac.kr
The increasing problem of antibiotic resistance among pathogenic bacteria requires development of new antimicrobial agents. For the purpose of this study, a cDNA encoding hinnavin Ⅱ-α-melanocyte stimulating hormone (hin/MSH) hybrid was chemically synthesized, annealed, and then cloned into transfer vector pBacPAK 9 for expression in Sf21 insect cells. Recombinant hin/MSH (rhin/MSH) hybrid was efficiently produced in baculovirus expression vector system (BEVS) as a hybrid peptide. The antibacterial activity of the rhin/MSH hybrid was compared to that of the recombinant hinnavin Ⅱ (rhin), using inhibition zone and overlay assay.
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