E fficacy of Iranian isolate of Metarhizium anisopliae (Metschnikof.) Sorokin against Russian wheat aphid Diuraphis noxia (Mordvilko) (Homoptera: Aphididae) on the laboratory condition
2010
Mohammadipour, A., Plant Pests and Diseases Reserch Institute | Ghazavi, M, Plant Pests and Diseases Reserch Institute | Bagdadi, A., Payam Noor University of Tehran Provience, Tehran- Iran
In this study, two isolates of the entomopathogenic fungus, Metarhizium anisopliae, obtained originally from red palm weevil (Rhynchophorus ferrugineus) and a beetle (Parandra caspica) have selected and order to sporulation these isolates cultured on SDA medium for tests. 120-150 adult Russian wheat aphid were treated with conidial concentrations of 1×105 and 1×10 6 spore/insect and 5 mlit suspension of each concentration prepared by spraying method. Then treated Aphid were placed in sterilized Plexiglas's Petri containing wheat, water & paper. Motality was recorded daily for 10 days. Cumulative percentage corrected for corresponding control. Data were analyzed by softwares PreProbit (1998-200) and Curve Expert1.3 to determine LC50 & LT50.
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